ImpARAS focuses on physical and chemical properties of proteins that may influence the sensitization (the formation of IgE) and are therefore a valuable parameter to predict the allergenicity of a protein. For example, protein-lipid interaction and stability in the gastro intestinal tract influences the bioavailability of the protein in the intestine and thus the presentation to the immune cells. Furthermore, the matrix of the food compound (e.g. presence of sugars and lipids in the food products) may protect the allergen from digestion or may even modulate interactions with certain cells involved in the immune cells. In that case, more or less intact protein will reach the intestine. It can be envisioned that other physical chemical parameters like amino acid composition, 2D/3D structure, thermal stability, matrix and post-translational modifications might influence the biological activity of proteins. Another important topic is how allergens can be purified from processed and non-processed products, without losing the physical and chemical properties as seen in the complete products and how to deal with aspects such as the presence of LPS and other contaminations.
Working Group 1 will discuss which physical and chemical properties of proteins may correlate with allergenicity and how to measure these. Next to this the effect of processing and matrix on protein structure and bioavailability will be discussed and investigated in the literature. A (generic) strategy for allergen purification and how to determine the purity will be developed. The contamination of for instance LPS within the samples will be taken into account in the purification protocol. Strategies to perform specific and efficient quantification of native as well as modified proteins in different conditions (such as cell culture media) will be discussed.
Representatives is this group are food scientists, protein experts, analytical chemists.
WG 1 LEADER
DR. GABRIEL MAZZUCCHELLI
University of Liege
Allee de la Chimie
Mass Spectrometry Laboratory
WG 1 DEPUTY LEADER
PROF. KARIN HOFFMANN-SOMMERGRUBER
Medical University of Vienna
Waehringer Guertel 18-20